These spatial layers were created to provide estimates of non-native species invasion risk across the contiguous United States based on proximity to human population centers and transportation corridors, and proximity to known locations of non-native species. To calculate the human transport risk layer we estimated the proximity to human population centers, transportation corridors, and speed of movement across the landscape. To calculate invasion risk based on known locations of non-native species, we gathered over 30 million records of non-native species occurrences across the contiguous United States from online databases to create nationwide maps of non-native species richness by species type: amphibians, fish, invertebrates, mammals, mollusks, plants, and reptiles.