This data release includes the results of state agency led electrofishing surveys conducted in lotic habitats in six states in the Northeastern U.S.: Maine, New Hampshire, Vermont, Massachusetts, Connecticut, and Rhode Island. The following state agencies collected the electrofishing data: Massachusetts Division of Fisheries & Wildlife, Vermont Department of Fish and Wildlife, Vermont Department of Environmental Conservation, Maine Department of Inland Fisheries & Wildlife, Rhode Island Department of Environmental Management, Connecticut Department of Energy and Environmental Protection, and New Hampshire Department of Fish and Game. The survey results are consolidated to a Hydrologic Unit Code (HUC)12 scale and includes surveys from November 1949 through December 2021 with most surveys occurring between 1985 and 2021. Fifty-three species and 24,553 surveys are represented in these data.