USGS scientists acquired aerial imagery from unmanned aerial vehicles (UAV) to map sagebrush at 25 sites in the Northern Great Plains during June 2023. Data include imagery with 1.5-centimeter (cm) resolution for each 300x300 meter (m) site and imagery at 0.75 cm for each of 10 to 12, 5x5 m plots within each site, and predicted areas of sagebrush for each site from models that classify cover types in imagery. Supporting data include training data, model variables, and polygons for plots used in validation. These sagebrush maps were developed to train satellite imagery for mapping over larger areas.