This study evaluated the use of dissolved carbon dioxide as a control method for juvenile red swamp crayfish (Procambarus clarkii). Four concentrations of CO2 and a control were administered to 18 test tanks over a 96-hour period. Red swamp crayfish were observed for behavioral changes during the exposure and were assessed for mortality 24 hours post treatment. Datasets include daily care, water quality, biometric, behavioral observation, flow rate, and CO2 titration data from one trial. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.