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Spatial Extent of Data

USGS Data Source

USGS Thesaurus Keywords

invasive species

ISO 19115 Topic Category

biota

Other Subject Keywords

Phragmites australis, gene silencing, gene expression, RNA interference

Place Keywords

Great Lakes, North America

Quantitative polymerase chain reaction data describing target gene silencing in the invasive common reed Phragmites australis subsp. australis using a leaf cutting bioassay

These data describe layered double hydroxide (LDH) mediated delivery of novel gene silencing agents (GSAs) in leaf tissue of the invasive common reed <i>Phragmites australis</i> ssp. <i>australis</i>. Double-stranded and hairpin RNAs were designed and synthesized to target paralogous genes coding for phytoene desaturase (PDS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). A leaf cutting bioassay was developed to rapidly screen GSAs for effective gene silencing action. Reduced expression of <i>PDS</i> and <i>EPSPS</i> were then characterized using reverse transcription quantitative PCR (RT-qPCR), the results of which are presented herein. Related sequences describing target genes from experimental plants are available via the National Center for Biotechnology Information's GenBank database (accession IDs: PQ634570, PQ634571, PQ634572, PQ634573).

Get Data and Metadata
Author(s) Seung Ho Chung, Kurt P Kowalski orcid, Alyxandra S Cicerrella, P.U. Ashvin Iresh, Pramod Pantha, Maheshi Dassanayake orcid
Publication Date 2025-04-10
Beginning Date of Data 2022-02-08
Ending Date of Data 2024-05-16
Data Contact
McKenzie Smith
DOI https://doi.org/10.5066/P1UMSYQY
Citation Chung, S.H., Kowalski, K.P., Cicerrella, A.S., Iresh, P.A., Pantha, P., and Dassanayake, M., 2025, Quantitative polymerase chain reaction data describing target gene silencing in the invasive common reed Phragmites australis subsp. australis using a leaf cutting bioassay: U.S. Geological Survey data release, https://doi.org/10.5066/P1UMSYQY.
Metadata Contact
Great Lakes Science Center
Metadata Date 2025-04-10
Related Publication
Citations of these data No citations of these data are known at this time.
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License http://www.usa.gov/publicdomain/label/1.0/
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Harvest Source: ScienceBase
Harvest Date: 2026-04-24T04:52:10.684Z