The Missouri Stream Conservation and Connectivity Prioritization Tool contains data tabulating costs to traverse between 10,357 origin and 50,135 destination stream reaches throughout the Central Plains and Ozarks aquatic subregions in Missouri. These data are intended to be used in the Missouri Stream Conservation and Connectivity Prioritization Tool and are not suitable stand-alone products outside of the context of the application. Cost tables listed here include watersheds that are directly connected to other watersheds within the state of Missouri and generally represent the northern 66% of the state. These watersheds are grouped together because they represent a continuous network of streams within the state. This represents 89 of the total 109 cost tables. Additional support files include a list of files provided on this landing page (A_DataDictionary_FileList_ParentItem.csv); a description of navigation files and their fields (A_DataDictionary_NavigationFileFields.csv), a description of cost table fields (A_DataDictionary_CostTableFields.csv), and a list of Missouri fish species and associated functional ecology (B_Species_List_AUTECOLOGY_2_FishGroups_DataRelease.csv).