As part of the larger Great Lakes Cooperative Science and Monitoring Initiative (CSMI) , the U.S. Geological Survey Mercury Research Laboratory (MRL) completed a binational assessment partnering with the U.S. Environmental Protection Agency (US EPA), Environmental Climate Change Canada (ECCC), Michigan-DNR (MI-DNR), University of Minnesota-Duluth Natural Resources Research Institute (UM-NRRI), and the National Oceanographic and Atmospheric Association (NOAA), to assess contaminant concentrations within seston, mussels, preyfish, waters, and surface sediments within Lake Huron. All matrices were assessed for mercury and methylmercury concentrations to examine spatial trends of mercury within the lakes. Sediments were also analyzed for total mercury stable isotope composition to approximate sources of mercury contamination to the lake.