Data include total nitrogen and carbon, carbon species, total metal, and particle-size analyses of soft streambed sediment from Little Flatrock Creek, Paulding County, Ohio. These data will be used in a sediment-source mixing model to attribute the proportional contribution of individual sources to suspended sediment in the basin. Source samples were identified as one of four land-cover types: cropland (corn, soybean, hay, and wheat; sites included a mix of conventional and conservation tillage), roads, preserved forest, and streambanks along agricultural and non-agricultural channels.