This data release includes physical and chemical data for streambed-sediment samples collected at 7 locations in the Little Flatrock Creek basin in Paulding County, Ohio during July 2019. Data include total nitrogen and carbon concentrations, carbon species, total concentrations for 41 metals, and particle-size analysis; not all samples had enough for all analyses. Source samples were identified as one of four land-cover types: cropland (corn, soybean, hay, and wheat; sites included a mix of conventional and conservation tillage), roads, preserved forest, and streambanks. All reaches had cropland on at least one side of the stream. These data will be used for source attribution in order to quantify the proportional contribution of individual sources to streambed sediment in the basin. The objective of "fingerprinting" these samples was to identify the potential contribution of surrounding land use to what is stored in the channel upstream from the gage.