These data include aerobic scope and thermal tolerance measurements from two cisco (Coregonus artedi) populations to evaluate performance and potential adaptation to warming Great Lakes habitats. Cisco come from two stock sources: 1) a northern latitude Lake Huron stock collected as gametes from Les Cheneaux Islands, MI, USA in 2015 and raised in a USGS aquaculture lab in Ann Arbor, MI, and 2) a southern latitude stock from Crooked Lake, Noble County, IN, USA collected as adults from the wild in 2021 and 2022 and brought to Ann Arbor, MI. Data include three primary datasets. "Aerobic Scope Compiled Data" provides measures of cisco (Coregonus artedi) metabolic rates from 30L swim chamber respirometry trials (n=88). "Acute Thermal Tolerance" provides measures of short term cisco tolerance to elevated temperatures within a 185L swim chamber. Cisco that did not lose equilibrium in the acute thermal tolerance experiments were reserved for the "Chronic Thermal Tolerance" which provides measures of long term cisco tolerance to elevated temperatures in recirculating aquaculture systems. At the end of each of the three experiments cisco length and weight were recorded.