This dataset includes benthic algae community composition data from three sites in the upper White River Basin, Colorado, in 2022. Benthic algae were identified as genus or species, and biomass and biovolume were provided for taxa.U.S. Geological Survey completed data collection in upper White River Basin in cooperation with the White River Conservation District. Data collection was funded by the U.S. Geological Survey Water Mission Area as part of the Next Generation Water Observing System Upper Colorado River project.