These data from Evaluation and Review of Ecology-Focused Stream Studies to Support Cooperative Monitoring, Fountain Creek Basin, Colorado were used to describe temporal trends in invertebrate communities in the basin. Invertebrate data were collected at U.S. Geological Survey (USGS) sites between 1985 and 2022. Datasets include invertebrate frequency of occurrence, invertebrate tolerance index values, invertebrate multi-metric index, New Zealand mudsnial counts, and list of invertebrate species collected.