This dataset represents the locations of experimental plots that were used during the Phragmites Bioherbicide 2023 field experiment. To meet the criteria for selection, a site must have relatively dry soil with no standing water, have two or more large monotypic stands of Phragmites australis, and be easily accessible by vehicle. Experimental plots were created at these sites and one of four treatments were applied to each plot involving either tap water or bioherbicide.