In June 2017, Lake Sturgeon were observed spawning in Fall Creek, a tributary to Cayuga Lake, NY. The spawning site was just downstream of Ithaca Falls, the first impassible barrier. Data were collected to quantify the stream habitat use by the Lake Sturgeon. These were the first sturgeon observed spawning in a Cayuga Lake tributary recorded in the New York Department of Environmental Conservation historical fish records. Habitat and egg deposition data were collected at 10 stations on 15 cross stream transects, spaced at least 10 m apart. Eggs were collected with using 7 cm diameter core sampler at least 3 stations on each transect. Water depth, stream velocity, and substrate composition data were collected at each station. Eggs were counted, identified, and determined to be alive or dead at Tunison Laboratory of Aquatic Science. These data will allow the quantification of Lake Sturgeon microhabitat selection for egg deposition during the spawning run.