This data release presents calculated accumulated wastewater (ACCWW, as a percent of total streamflow) values for 43 National Hydrologic Dataset Version 2.1 (NHDPlus V2.1) stream segments coinciding with long-term smallmouth bass sampling locations (Table 1) in the Shenandoah River Watershed (encompassing parts of Virginia and West Virginia, USA). Values are calculated for quarter-year (Quarter 1 [Q1], January - March; Quarter 2 [Q2], April - June; Quarter 3 [Q3], July-September; Quarter 4 [Q4], October-December) time scales (Table 2) and annual time scales (Table 3) for years 2000 to 2018. Estimates at a stream segment represent the combined total upstream wastewater discharges as well as direct discharges into the stream segment. Any users of these data should review the entire metadata record and the associated manuscript (see Larger Work Citation). See 'Distribution Liability' statements for more information.