Water-quality samples were collected at 49 surface water sites covering a range of land uses across the state of Iowa starting the summer of 2022 and continuing into the spring of 2023 by staff from the U.S. Geological Survey Central Midwest Water Science Center. Samples were submitted to the National Water Quality Laboratory in Denver, Colorado, and were analyzed for 32 per- and polyfluoroalkyl substances. Additional samples for N-(1,3-Dimethylbutyl)-N’-phenyl-p-phenylenediamine (6PPD) and 6PPD-Quinone were sent from a subset of the sites to the Organic Geochemistry Research Laboratory in Lawrence, Kansas.