4+ year old cisco (Coregonus artedi) pre-spawn and spawning/near-spawning behavioral data were collected and compiled at the Tunison Laboratory of Aquatic Science in Cortland, NY USA, between August-October 2021 and November-December 2021. Data are comprised of fish reactions in choice flume video data (attractance/avoidance). Cisco were exposed/not exposed to an imprinting chemical (amino acids: alanine, histidine, morpholine, proline, and serine) through early life stages, and later tested based on their treatment to see how individuals reacted to the presence of exposure chemicals during subsequent life stages. These data show how the adult stage cisco of this study ((non exposed (control) and treatment (amino acid exposed)) react to timed exposure of morpholine dilution at the 4+ year life stages.