‘Ōpe‘ape‘a (Hawaiian hoary bats; Lasiurus semotus) were surveyed at 23 sites on Hawaiʻi Island from 33 to 2,341 m elevation from May 2018 to August 2021. Bats were captured using mist nets; once detangled from mist nets, bats were secured in cloth holding bags for up to 10 min before collecting age, sex, reproductive condition, mass, forearm length, and biological samples and marking individuals with split ring bands and attaching very high frequency (VHF) radio transmitters. We captured 138 uniquely identified individuals (37 female, 101 male), of which 10 were recaptured (4 female, 6 male) and an additional 10 were ensnared in nets and escaped, for a total of 158. This data file includes data related to individual bats captured including sex, age and morphometrics of captured individuals, samples collected, markings applied, transmitter frequency, dates and generalized locations of capture.