The study was designed to assess the strength of the innate linkage of substrate and food in foraging by early juvenile Pallid and Shovelnose Sturgeon and their hybrids and adult Sicklefin and Shoal Chub. The data include information on the fish weights throughout the duration of the experiment as well as observed fish behavior data. Wet weights from fish were recorded to monitor feeding consumption and the behavior data was generated in response to watching the fish during feeding. Information found in the fish weights data table includes the fish name, the tank number, the food treatment given, the light treatment given, the week of the trial, the date when the weight was taken, the substrate material, where food was delivered, and lastly the fish weight in grams. Information found in the fish behavior data table includes the observed behavior of the fish, a numerical identifier given to the observation, the fish name, the week of the experiment, the food treatment given, the light treatment given, the substrate material, where food was delivered, the time in seconds an observed behavior started, the date of the observed behavior, the time the observation period started, and lastly the tank number.