This is the qPCR and loop-mediated isothermal amplification (LAMP) data in support of the article "Validation of a portable eDNA detection kit for invasive carps". There are four types of data contained in five files including: limit of detection analysis for each method (qPCR and LAMP), qPCR analysis and LAMP analysis of eDNA samples collected over time from ponds containing 3 or 33 Grass Carp (Ctenopharyngodon idella) mixed with thousands of baitfish. The standards and controls data collected with the eDNA sample analysis are split into separate files for each method to simplify the statistical analysis of the eDNA sample data.