Bees were collected in 24 fields across eastern Iowa in summer 2019. This data collection was part of a pesticide study funded by the USGS Ecosystems Mission Area- Environmental Health Program. Bees were collected using the sweep net method and then were immediately placed on dry ice in the field. Bees were kept frozen to prevent degradation. In the lab, each wild bee was photographed from one or more angles using an AmScope microscope fitted with an MU1400 digital camera at 20x magnification. Bees were then morphologically identified based on the images. All images were checked for quality control before they were archived on this site. This data release includes 1) a txt file with bee identifications and image names, 2) zipped images of the bees, and 3) a text file with alternative text for each image.