U. S. Geological Survey (USGS) scientists completed a stream survey campaign from the 26th of July to the 13th of August in 2021 at thirty 100-meter reaches within the Shenandoah Valley throughout Virginia and West Virginia. In each stream reach, the following survey measurements were made or attempted: three representative channel cross sections, water surface elevation at the top and bottom of each reach, and longitudinal thalweg point data. The survey data will be used with additional geomorphological measurements and habitat information to assess habitat quality of these Shenandoah Valley streams. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.