Geophysical measurements were collected by the U.S. Geological Survey (USGS) at five sites in Interior Alaska in September 2021 for the purposes of imaging permafrost structure and quantifying variations in subsurface moisture content in relation to thaw features. Electrical resistivity tomography (ERT) measurements were made along transects 110 - 222 m in length to quantify subsurface permafrost characteristics. ERT transects were collected across a fireline boundary within the Bonanza Creek Long Term Ecological Research (LTER) site where repeat measurements have been made since 2014; across and adjacent to two thermokarst lakes, Vault Lake and Goldstream Lake; and along two profiles at the North Star golf course in Fairbanks, Alaska. Models of electrical resistivity produced from these data revealed the distribution of frozen and thawed soil to depths of 10-40 m below the surface. Manual permafrost-probe measurements of thaw depths were collected at set intervals along each ERT transect used for comparison to the geophysical measurements, except at the North Star golf course where shallow permafrost was absent.