This data set, compiled by the USGS Lake Erie Biological Station, provides near-bottom measurements of temperature and dissolved oxygen for the Central Basin of Lake Erie. Data were recorded by self-contained environmental data loggers (PME, Inc., MiniDOT data loggers). The data loggers were deployed to collect data during mid-May 2021 through late October 2021 to better understand how hypoxia develops and influences fish habitat quality during lake stratification. The data loggers were deployed on acoustic telemetry receivers in a network designed to record tagged fish movements (Great Lakes Acoustic Telemetry Observation System, GLATOS). Additional years of data collection are planned, and data release will be updated as necessary.