Actual evapotranspiration (ETa) rates from the Operational Simplified Surface Energy Balance (SSEBop) method, before and after bias corrections, are presented for basins located wholly or partially within the St. Johns River Water Management District (SJRWMD). The SSEBop annual rates are provided at about a one square-kilometer scale from 2000 to 2017. Annual ETa rates calculated from the application of the water-balance method (wbETa) to 19 basins in the SJRWMD and annual land-use ETa (luETa) rates calculated from monthly average crop coefficient ratios are also provided in this data set. A GIS shapefile provides land-use type and annual ETa rate for each year of record. Data are tabulated in spreadsheets for each basin and are referred to by the name between parenthesis: Fort Drum Creek basin (02231342), Blue Cypress Creek basin (02231396), St. Johns River at Melbourne basin (02232000), Pennywash Creek basin (02232155), Apopka Beauclair Canal basin (02237700), Ocklawaha Moss Bluff basin (02238500), Orange Creek at Orange Spring basin (02243000), St. Johns River at Buffalo Bluff basin (02244040), Haw Creek above Russell Landing basin (02244333), Tomoka River basin (02247510), Turkey Creek basin (02250030), St. Johns River near Christmas basin (02232500), Econlockhatchee River basin (02233484), Wekiva River basin (02235000), Deep Creek basin (02245260), Black Creek basin (02245500), North Fork Black Creek basin (02246000), Eau Gallie River basin (02249007), and South Prong at St. Sebastian River basin (02251000). Inflows and outflows from various sources in the water-balance equation used to calculate annual average wbETa rates for each basin in the SJRWMD area are presented in this dataset.