The data include concentrations of current use pesticides in tissues of larval wood frog (Lithobates sylvaticus) and spotted salamander (Ambystoma maculatum) and the presence of ranavirus in wood frogs and spotted salamanders from three northeastern National Wildlife Refuges sampled in 2013 and 2014. The data also include estrogenicity, protein phosphatase 2A inhibition and a suite of 15 major and minor elements in sediment screened using portable X-Ray Fluorescence. The data include sediment and tissue samples collected from 16 wetlands at the Patuxent Research Refuge (PRR) in central Maryland, USA, 15 wetlands at the Assabet River and Oxbow National Wildlife Refuges (EMASS) in eastern Massachusetts, USA, and nine wetlands at the Chesapeake and Ohio Canal National Historic Park (CHOH) near the border of Washington DC and Maryland, USA.