A Geographic Information System (GIS) shapefile and summary tables of irrigated agricultural land-use are provided for the fourteen counties that are fully or partially within the Suwannee River Water Management District, Florida compiled through a cooperative project between the U.S Geological Survey and the Florida Department of Agriculture and Consumer Services, Office of Agricultural Water Policy. Information provided in the shapefile includes the location of irrigated lands that were verified during field trips that started in January 2020 and concluded in December 2020, and the crop type, irrigation system type, and primary water source used. A map image of the shapefile is provided. Previously published estimates of irrigation acreage for years since 1982 are included in summary tables.