Through the North American Bat Monitoring Program, Bat Conservation International and U.S Geological Survey (USGS) provided technical and science support to assistance in U.S. Fish and Wildlife Service Species Status Assessment (“SSA”) for the northern long-eared bat (Myotis septentrionalis), little brown bat (Myotis lucifugus), and tri-colored bat (Perimyotis subflavus). USGS facilitated the SSA data call providing data archival for repeatable and transparent analyses, provided statistical support to assess the historical, current, an future population status for each of the three species, and developed a demographic projection tool to evaluate future viability of each species under multiple threat scenarios. We assessed population trends from count surveys of wintering colonies at hibernacula for these three bat species. Winter colony counts were downloaded from the database of the North American Bat Monitoring Program (U.S. Geological Survey North American Bat Monitoring Program. Accessed 2020-12-01. NABat Request Number 12. Database Version v5.4.0).