Fish collection data for surveys made at 5 localities in Flat Creek, Dawson County GA, on 6 dates that spanned a 4 to 18.5-month period following a catastrophic fish kill caused by a chemical spill near the headwater origin on Flat Creek. The data are: locality descriptions, water depth and velocity measurements made during fish sampling, and tabulated numbers and range in body length of individuals observed, by collection date and locality.