Archived plasma from fish health monitoring sites within the Chesapeake Bay watershed was analyzed for 13 perfluoroalkyl compounds. Smallmouth bass were collected from two sites in the Potomac River drainage (Antietam Creek, Maryland and South Branch Potomac, West Virginia) and two in the Susquehanna River drainage (West Branch Mahantango Creek, Pennsylvania and Pine Creek, Pennsylvania). Site and date of collection, length, weight and age of each adult smallmouth bass were documented. Four compounds were detected in every plasma sample, while two additonal compounds were only occasionally observed at low concentrations.