This dataset contains all input parameters required to perform integrated step selection analysis (iSSA) to quantify marine turtle habitat selection relative to marine protected areas throughout coastal Florida and the Gulf of Mexico. The iSSA is a flexible approach to compare the environmental attributes of observed steps (the linear segment connecting two consecutive observed positions of an animal) against a group of alternative steps taken from the same starting location. Information contained in the dataset includes original PIT (passively induced transponder, a unique identifier for each turtle), step ID, step length and log of the step length, turning angle and cosine of turning angle, start and end date time of each step, protected status of location of each step ID (unprotected, multi-use or no-take, water depth (in meters), and chlorophyll concentration (in mg/m3).