The Grand Calumet River (GCR), located in northern Indiana, is contaminated due to a wide range of historical industrial activities. Short-term and long-term sediment exposure bioassays with the amphipod Hyalella azteca, the midge Chironomus dilutus, and the mussel Lampsilis siliquoidea were conducted with samples collected in 2013, 2015, and 2017, from up to 26 sites, including both remediated, non-remediated and relatively uncontaminated reference sites. The responses (survival, growth, reproduction and/or emergence) of test organisms to the exposure bioassays are presented here.