This dataset includes a subset of previously released pesticide data (Morace and others, 2020) from the U.S. Geological Survey (USGS) National Water Quality Assessment Program (NAWQA) Regional Stream Quality Assessment (RSQA) project and the corresponding hazard index results calculated using the R package toxEval, which are relevant to Mahler and others, 2020. Pesticide and transformation products were analyzed at the USGS National Water Quality Laboratory in Denver, Colorado. Files are grouped as pesticides (parent compounds), transformation products (degradate compounds), compounds with no Acute Invertebrate (AI) benchmarks, compounds with no Acute Non-Vascular Plant (ANVP) benchmarks, and compounds not evaluated through the toxEval R program. See Morace and others, 2020 for corresponding quality assurance or quality control data.