This U.S. Geological Survey (USGS) Data Release provides time-lapse color photography collected from three sites at Milford Lake, Kansas, during June through November 2017 and June through November 2018. Photographic images are displayed using a .mp4 common multimedia format. Time-lapse cameras were used to visually capture cyanobacterial accumulations and movement throughout Milford Lake, Kansas, at five-minute intervals (South Causeway Camera and Fort Riley Marina Camera) and at fifteen-minute intervals (Wakefield Camera). This dataset includes time-lapse photography collected at three short-term, fixed-site locations as part of a study to evaluate the temporal and spatial variability of nutrients and algal constituents in Milford Lake, Kansas.