Data were collected in 2017 by researchers at the USGS, USDA-ARS, and University of Wyoming on the food webs of plants, prairie dogs, arthropods, and birds in the Thunder Basin National Grassland. Data were collected from 87 sites in order to parameterize a structural equation model linking prairie dog impacts to changes in vegetation, arthropods, and birds. Abiotic information such as topographic wetness index, terrain roughness, and soil characteristics were estimated at the same set of plots in order to account for abiotic variation across the landscape.