Image and biometric data were collected for 22 species of fish from Great Lakes Tributaries in Michigan and Ohio, and the Illinois River for the purpose of developing a fish identification classifier. Data consists of a comma delimited spreadsheet that identifies image file names and associated fish identification number, common name, species code, family name, genus, and species, date collected, river from which each fish was collected, location of sampling, fish fork length in millimeters, girth in millimeters, weight in kilograms, and personnel involved with image collection. Biometric data are saved as .csv comma delimited format and image files are saved as .png file type.