The dataset “Glacier_National_Park_alpine_stream_sampling_watersheds,_2011-2013.shp” is a GIS shapefile of the drainage areas of each of the 129 alpine and glacial aquatic macroinvertebrate collection sites included in an analysis to test the prediction that glacier retreat reduces biodiversity in mountain ecosystems through the loss of uniquely adapted meltwater stream species in Glacier National Park, USA. In conjunction with the dataset “Minimum snow and ice coverage for GNP, 2003-2015”, this dataset was used to calculate the proportion of contemporary ice and snow coverage for each watershed drainage area.