Low-altitude digital images were taken over an area of the Potomac River in Shepherdstown, West Virginia using 3DR Solo unmanned aircraft systems (UAS) on October 21, 2019. The imagery was collected for the purpose of evaluating UAS assessment of river habitat data such as water depth, substrate type, and water clarity. Some photographs contain black and white targets used as ground control points (GCPs), which were surveyed by a field crew with a high-precision (GNSS) Global Navigation Satellite System and/or containing internal post processing kinematic (PPK) GPS system. This data release contains the csv file containing the latitude and longitude coordinates, in Universal Transverse Mercator Zone 18N referenced to the North American Datum of 1983 (2011), of the ground control points.