Rapid advances in video technology are enabling new strategies for species abundance estimation. Here we provide estimates of fish abundance derived from video data collected in a series of stream pools in Shenandoah National Park, Virginia (n=41). Two 360-degree cameras were simultaneously used at each pool site where 15-minutes of underwater footage was collected. Environmental data are provided describing pool morphology, canopy cover, and fish cover (boulders, large woody debris, and undercut banks).