This data release includes physical and chemical data for samples from upland-source and streambank sampling sites in the Black Creek basin, Indiana. Data include total nitrogen and carbon concentrations, carbon species, total concentrations for 42 metals, and particle-size analysis. Source samples were identified as one of five land-use types: active cropland (corn, soybean, hay, and wheat), active horse pasture, roads, preserved forest, and streambanks of agricultural and non-agricultural channels. Source data were collected in August and September 2017. These data are used for source attribution in order to quantify the proportional contribution of individual sources to suspended sediment in the basin [Williamson et al. in prep].