Groundwater-quality data were collected from 648 wells as part of the National Water-Quality Assessment Project of the U.S. Geological Survey National Water-Quality Program and are included in this report. Most of the wells (514) were sampled from January through December 2016 and 60 of them were sampled in 2013 and 74 in 2014. The data were collected from seven types of well networks: principal aquifer study networks, which are used to assess the quality of groundwater used for public water supply; land-use study networks, which are used to assess land-use effects on shallow groundwater quality; major aquifer study networks, which are used to assess the quality of groundwater used for domestic supply; enhanced trends networks, which are used to evaluate the time scales during which groundwater quality changes; vertical flow-path study networks, which are used to evaluate changes in groundwater quality from shallow to deeper depths; flow path study networks, which are used to evaluate changes in groundwater quality from shallow to deeper depths over a horizontal distance; and modeling support studies, which are used to provide data to support groundwater modeling. Groundwater samples were analyzed for a large number of water-quality indicators and constituents, including major ions, nutrients, trace elements, volatile organic compounds, pesticides, radionuclides, and some special interest constituents (arsenic speciation, chromium [VI] and perchlorate). These groundwater quality data are tabulated in a U.S. Geological Survey Data Series Report DS-1124 which is available at https://dx.doi.org/XXXXXX and in this data release. Some data from environmental samples collected in 2013-14 and quality-control samples collected in 2012-15 also are included in the associated data release. Data from samples collected in 2016 are associated with networks described in this report and have not been published previously; data from samples collected between 2012 and 2015 are associated with networks described in previous reports in this data series (Arnold and others, 2016a,b, 2017a,b, 2018a,b). There are 23 data tables included in this data release and they are referenced as tables 1-13 and appendix tables 4.10-4.19 in the larger work citation. There are 36 tables that are part of the larger work citation; the 13 tables not included in the data release are summary tables derived from some of the other tables (tables 1.1, 2.2-2.3, 3.1, 4.1-4.9). A version of table 1 is included in both the text and data release. This compressed file contains 23 files of groundwater-quality data in ASCII text tab-delimited format and one corresponding metadata in xml format that describes all the tables and attributes.