This dataset is a collection of hyperspectral imagery profiles of 13 common algae associated with Harmful Algae Blooms (HAB). Data were retrieved from a hyperspectral microscope at, and with the cooperation of, the National Institute of Standards and Technology. Samples were collected from USGS water quality sampling efforts, and were also purchased from commercial vendors of biological materials. Data are shown in basic hyperspectral imagery form, transformed into the first derivative and corrected with a flat field algorithm to account for variations in locale lighting conditions.