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Spatial Extent of Data

USGS Data Source

ISO 19115 Topic Category

biota

Other Subject Keywords

PCR, Environmental DNA, detection, eDNA, invasive species

Place Keywords

School of the Environment, Washington State University, 100 Dairy Road, Pullman, Washington, 99164, Columbia Environmental Research Center, 4200 E New Haven Road, Columbia, Missouri, 65201, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, Colorado, 80521, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Peterborough, Ontario, K9L 0G2, Canada, Upper Midwest Environmental Sciences Center, 2630 Fanta Reed Road, La Crosse, Wisconsin, 54603, Engineers Research and Development Center, 3909 Halls Ferry Road, Vicksburg, Mississippi, 39180, Department of Biochemistry and Microbiology, University of Victoria, 3800 Finnerty Road, Victoria, British Columbia, V8P 5C2, Canada, Whitney Genetics Laboratory, 555 Lester Avenue, Onalaska, Wisconsin, 54650

Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data

This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis

Get Data and Metadata
Author(s) Christopher M Merkes orcid, Katy E Klymus orcid, Michael Allison, Caren S Goldberg, Caren Helbin, Margaret (Maggie) E. orcid, Craig A Jackson orcid, Richard Lance, Anna Mangan, Emy M Monroe, Antoineete Piagio, Joel P Stokdyk orcid, Chris Wilson, Cathy A Richter orcid
Publication Date 2019-07-23
Beginning Date of Data 2017-11-01
Ending Date of Data 2019-05-01
Data Contact
Christopher M Merkes
DOI https://doi.org/10.5066/P9AKHU1R
Citation Merkes, C.M., Klymus, K.E., Allison, M., Goldberg, C.S., Helbin, C., E., M.(., Jackson, C.A., Lance, R., Mangan, A., Monroe, E.M., Piagio, A., Stokdyk, J.P., Wilson, C., and Richter, C.A., 2019, Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data: U.S. Geological Survey data release, https://doi.org/10.5066/P9AKHU1R.
Metadata Contact
Christopher M Merkes
Metadata Date 2022-04-28
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License http://www.usa.gov/publicdomain/label/1.0/
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Harvest Source: ScienceBase
Harvest Date: 2024-12-27T21:29:05.330Z