Trends in nutrient fluxes and streamflow for selected tributaries in the Lake Erie watershed were calculated using monitoring data at 10 locations. Trends in flow-normalized nutrient fluxes were determined by applying a weighted regression approach called WRTDS (Weighted Regression on Time, Discharge, and Season). Site information and streamflow and water-quality records are contained in 3 zipped files named as follows: INFO (site information), Daily (daily streamflow records), and Sample (water-quality records). The INFO, Daily (flow), and Sample files contain the input data, by water-quality parameter and by site as .csv files, used to run trend analyses. These files were generated by the R (version 3.1.2) software package called EGRET - Exploration and Graphics for River Trends (version 2.5.1) (Hirsch and DeCicco, 2015), and can be used directly as input to run graphical procedures and WRTDS trend analyses using EGRET R software. The .csv files are identified according to water-quality parameter (TP, SRP, TN, NO23, and TKN) and site reference number (e.g. TPfiles.1.INFO.csv, SRPfiles.1.INFO.csv, TPfiles.2.INFO.csv, etc.). Water-quality parameter abbreviations and site reference numbers are defined in the file "Site-summary_table.csv" on the landing page, where there is also a site-location map ("Site_map.pdf"). Parameter information details, including abbreviation definitions, appear in the abstract on the Landing Page. SRP data records were available at only 6 of the 10 trend sites, which are identified in the file "site-summary_table.csv" (see landing page) as monitored by the organization NCWQR (National Center for Water Quality Research). The SRP sites are: RAIS, MAUW, SAND, HONE, ROCK, and CUYA. The model-input dataset is presented in 3 parts: 1. INFO.zip (site information) 2. Daily.zip (daily streamflow records) 3. Sample.zip (water-quality records) Reference: Hirsch, R.M., and De Cicco, L.A., 2015 (revised). User Guide to Exploration and Graphics for RivEr Trends (EGRET) and dataRetrieval: R Packages for Hydrologic Data, Version 2.0, U.S. Geological Survey Techniques Methods, 4-A10. U.S. Geological Survey, Reston, VA., 93 p. (at: http://dx.doi.org/10.3133/tm4A10).