The NABat sampling frame is a grid-based finite-area frame spanning Canada, the United States, and Mexico consisting of 10-km by 10-km (100-km2) grid cell sample units. This grain size is biologically appropriate given the scale of movement of most bat species, which routinely travel many kilometers each night between roosts and foraging areas and along foraging routes. A draw of sample units from a finite sampling frame using the GRTS design produces an ordered list of units such that any ordered subset of that list is also randomized and spatially balanced. This vector dataset is the individual grid-based sampling grid for Alaska and Canada at a 10x10km resolution.