These data contain biodata, net catch, and detection records for juvenile sea lamprey sampled in natural streams in Michigan and Quebec, Canada and those stocked into an artificial stream at the USGS Hammond Bay Biological Station for monitoring diel activity. During October 31 through November 9, 2011 scientists collected downstream migrating juvenile sea lamprey from the Little Carp River, Michigan, Lake Superior (46°50'6.34"N 88°28'57.58"W). Collections were permitted by the State of Michigan under the Michigan Department of Natural Resources Scientific Collectors Permit issued to U.S. Geological Survey, Hammond Bay Biological Station December 12, 2007; amended February 23, 2011. Between November 3, 2014 and December 15, 2014, scientists monitored lamprey downstream passage using passive integrated transponder (PIT) telemetry in Morpion Stream, Quebec, Canada, Lake Champlain (45°10'23.68"N 73° 2'16.98"W). During October 2014 through February 2015, scientists monitored movement activity of juvenile sea lamprey using PIT telemetry in an artificial stream located at the Hammond Bay Biological Station, Millersburg, Michigan. Collections from Morpion Stream, Quebec during 2014 were conducted under Quebec Ministere des Resources Naturelles permis a des fins scientifiques 2012-10-01-1436-16-SP to Ellen Marsden and a scientific collection permit from the Vermont Department of Fish and Wildlife. Fish care and protocols for fish holding, surgery, and tagging were conducted under a University of Vermont Institutional Animal Care and Use Committee, permit 13-017.