In 2017, the U.S. Fish and Wildlife Service, U.S. Geological Survey, and U.S. Army Corps of Engineers undertook a large-scale interagency field study to determine the influence of commercial barge vessels on the efficacy of the Electric Dispersal Barrier System (EDBS) in the Chicago Sanitary and Ship Canal (CSSC) in preventing fish passage. This study included a series of trials in which a tow, consisting of a tug vessel and fully-loaded barges, transited the EDBS in both upstream-bound (n = 65) and downstream-bound (n = 66) directions. Wall-mounted velocity probes were located at (41.6423629, -88.060329). The configuration of the barges for each run, and the time at which the bow and stern of the tow pass the wall-mounted velocity probes are provided in this Comma Separated Value (CSV) file.