This data release includes characteristics of wetland catchments associated with U.S. Fish and Wildlife conservation easement lands located in the Prairie Pothole Region of North Dakota. Characteristics include wetland catchment areas, slope length, land use, soil mapping unit, and slope grades of representative soils. County and ecoregion also are included. Summary data pertaining to lateral setback distances, or drainage setbacks, also are presented by county and soil mapping unit for a range of subsurface drainage system characteristics (i.e., drain pipe depth and diameter). Additionally, calculated variables used for data analyses and presentation in the manuscript associated with this data release are included.