The hydrologic response units (HRUs) and stream segments available here are for an application of the Precipitation Runoff Modeling System (PRMS) in the southeastern United States by LaFontaine and others (2019). Geographic Information System (GIS) files for the HRUs and stream segments are provided as shapefiles with attribute hru_id_1 identifying the HRU numbering convention used in the PRMS model and seg_id_gcp identifying the stream segment numbering convention used in the PRMS model. This GIS files represent the watershed area for an approximately 1.16 million square kilometer area of the southeastern United States. A total of 20,251 HRUs and 10,742 stream segments are used in this modeling application. LaFontaine, J.H., Hart, R.M., Hay, L.E., Farmer, W.H., Bock, A.R., Viger, R.J., Markstrom, S.L., Regan, R.S., and Driscoll, J.M., 2019, Simulation of Water Availability in the Southeastern United States for Historical and Potential Future Climate and Land-Cover Conditions: U.S. Geological Survey Scientific Investigations Report, 2019-5039, 83 p., https://doi.org/10.3133/sir20195039.