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Spatial Extent of Data

USGS Data Source

USGS Thesaurus Keywords

environmental DNA, molecular biology, parasites, fish

ISO 19115 Topic Category

biota

Other Subject Keywords

PCR, Tetracapsuloides bryosalmonae, Proliferative Kidney Disease

Place Keywords

Sypes Canyon, California, East Gallatin River, Wyoming, Montana, Idaho

Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples

We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.

Get Data and Metadata
Author(s) Adam J Sepulveda orcid
Publication Date 2017
Beginning Date of Data 2017-03-05
Ending Date of Data 2017-03-05
Data Contact
Adam J Sepulveda
DOI https://doi.org/10.5066/F7C53JDW
Citation Sepulveda, A.J., 2017, Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples: U.S. Geological Survey data release, https://doi.org/10.5066/F7C53JDW.
Metadata Contact
Adam J Sepulveda
Metadata Date 2020-08-26
Related Publication
Citations of these data No citations of these data are known at this time.
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License http://www.usa.gov/publicdomain/label/1.0/
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Harvest Source: ScienceBase
Harvest Date: 2021-11-19T04:42:53.907Z